Cell-free DNA is purified from plasma collected in Streck cell-free DNA BCT® tubes. Cell-free DNA extracted from plasma is used as the template in a next generation sequencing assay. Based on a proprietary algorithm that uses the known population frequencies of the SNPs sequenced and expected distributions of alleles, the percentage of the cell-free DNA that is derived from the transplanted organ (dd-cfDNA) is computed (Grskovic et al., J Mol Diagn, 2016).
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